It is recognized now that a variety of real-life phenomena ranging from diffusion of cold atoms to the motion of humans exhibit dispersal faster than normal diffusion. Levy walks is a model that excelled in describing such superdiffusive behaviors albeit in one dimension. Here we show that, in contrast to standard random walks, the microscopic geometry of planar superdiffusive Levy walks is imprinted in the asymptotic distribution of the walkers. The geometry of the underlying walk can be inferred from trajectories of the walkers by calculating the analogue of the Pearson coefficient.
Veronika Fitz,
Jaeoh Shin,
Christoph Ehrlich,
Lucas Farnung,
Patrick Cramer,
Vasily Zaburdaev,
Stephan W. Grill
Proceedings of the National Academy of Sciences of the United States of America
113
(45)
12733-12738
(2016)
| Journal
In eukaryotes, gene expression depends on chromatin organization. However, how chromatin affects the transcription dynamics of individual RNA polymerases has remained elusive. Here, we use dual trap optical tweezers to study single yeast RNA polymerase II (Pol II) molecules transcribing along a DNA template with two nucleosomes. The slowdown and the changes in pausing behavior within the nucleosomal region allow us to determine a drift coefficient,., which characterizes the ability of the enzyme to recover from a nucleosomal backtrack. Notably, chi can be used to predict the probability to pass the first nucleosome. Importantly, the presence of a second nucleosome changes chi in a manner that depends on the spacing between the two nucleosomes, as well as on their rotational arrangement on the helical DNA molecule. Our results indicate that the ability of Pol II to pass the first nucleosome is increased when the next nucleosome is turned away from the first one to face the opposite side of the DNA template. These findings help to rationalize how chromatin arrangement affects Pol II transcription dynamics.
Reaction front propagation of actin polymerization in a comb-reaction system
We develop a theoretical model of anomalous transport with polymerization-reaction dynamics. We are motivated by the experimental problem of actin polymerization occurring in a microfluidic device with a comb-like geometry. Depending on the concentration of reagents, two limiting regimes for the propagation of reaction are recovered: the failure of the reaction front propagation and a finite speed of the reaction front corresponding to the Fisher-Kolmogorov-Petrovskii-Piscounov (FKPP) at the long time asymptotic regime. To predict the relevance of these regimes we obtain an explicit expression for the transient time as a function of geometry and parameters of the experimental setup. Explicit analytical expressions of the reaction front velocity are obtained as functions of the experimental setup. (C) 2016 Elsevier Ltd. All rights reserved.
A pH-driven transition of the cytoplasm from a fluid- to a solid-like
state promotes entry into dormancy
Matthias Christoph Munder,
Daniel Midtvedt,
Titus Franzmann,
Elisabeth Nueske,
Oliver Otto,
Maik Herbig,
Elke Ulbricht,
Paul Mueller,
Anna Taubenberger, et al.
Cells can enter into a dormant state when faced with unfavorable conditions. However, how cells enter into and recover from this state is still poorly understood. Here, we study dormancy in different eukaryotic organisms and find it to be associated with a significant decrease in the mobility of organelles and foreign tracer particles. We show that this reduced mobility is caused by an influx of protons and a marked acidification of the cytoplasm, which leads to widespread macromolecular assembly of proteins and triggers a transition of the cytoplasm to a solid-like state with increased mechanical stability. We further demonstrate that this transition is required for cellular survival under conditions of starvation. Our findings have broad implications for understanding alternative physiological states, such as quiescence and dormancy, and create a new view of the cytoplasm as an adaptable fluid that can reversibly transition into a protective solid-like state.
Kontakt
Abteilung Immunophysik Prof. Vasily Zaburdaev Principal Investigator
Max-Planck-Zentrum für Physik und Medizin Kussmaulallee 2 Raum 02.116 91054 Erlangen 09131 8284 102
