Publikationen Abteilung Neuronale Mechanik

Superhydrophobicity is generally considered to be a thermodynamically stable wetting state. The stability of the plastron (the thin air film separating the substrate from the water in the superhydrophobic state) was studied in underwater experiments. The plastron exhibited a rapid decay after a well defined onset time, which was found to be dependent on the immersion depth. The plastron decay is explained in terms of a model, which is based on confocal microscopy measurements. The limited underwater plastron stability explains the rarity of permanently submerged superhydrophobic surfaces in nature and limits their scope for commercial applications.

Cells are able to detect and respond to mechanical cues from their environment. Previous studies have investigated this mechanosensitivity on various cell types, including neural cells such as astrocytes. In this study, we have carefully optimized polyacrylamide gels, commonly used as compliant growth substrates, considering their homogeneity in surface topography, mechanical properties, and coating density, and identified several potential pitfalls for the purpose of mechanosensitivity studies. The resulting astrocyte response to growth on substrates with shear storage moduli of G' = 100 Pa and G' = 10 kPa was then evaluated as a function of coating density of poly-D-lysine using quantitative morphometric analysis. Astrocytes cultured on stiff substrates showed significantly increased perimeter, area, diameter, elongation, number of extremities and overall complexity if compared to those cultured on compliant substrates. A statistically significant difference in the overall morphological score was confirmed with an artificial intelligence-based shape analysis. The dependence of the cells' morphology on PDL coating density seemed to be weak compared to the effect of the substrate stiffness and was slightly biphasic, with a maximum at 10-100 mu g ml(-1) PDL concentration. Our finding suggests that the compliance of the surrounding tissue in vivo may influence astrocyte morphology and behavior.

PURPOSE: To test whether Müller glial cells sense, and respond to, mechanical tension in the retina.<br>METHODS: A device was designed to stretch the retina at right angles to its surface, across retinal layers. Pieces of retina were mounted between two hollow tubes, and uniaxial force was applied to the tissue using a micrometer-stepping motor. Müller cells were selectively stained with the fluorescent, calcium-sensitive dye X-Rhod-1 and were monitored in real time during retinal stretch in vitro. Immunohistochemistry was used to study protein levels and activation of intracellular pathways in stretched retinas.<br>RESULTS: Müller cells responded acutely with transient increases in fluorescence during stretch, indicative of increased intracellular calcium levels. All the Müller cells elongated uniformly, and there was no apparent difference between retinal layers in resistance against mechanical deformation. After stretch, Müller cells showed fast activation of extracellular signal-regulated kinase (after 15 minutes), upregulation of transcription factor c-Fos (after 1 hour), and basic fibroblast growth factor (after 3 hours). No changes in intermediate filament protein expression were observed in Müller cells up to 3 hours after stretch.<br>CONCLUSIONS: A novel technique was developed for real-time monitoring of Müller cells during retinal stretch, which allowed the identification of Müller cells as a mechanoresponsive cell type. Mechanical stress triggers molecular responses in Müller cells that could prevent retinal damage.

The mechanical properties of tissues are increasingly recognized as important cues for cell physiology and pathology. Nevertheless, there is a sparsity of quantitative, high-resolution data on mechanical properties of specific tissues. This is especially true for the central nervous system (CNS), which poses particular difficulties in terms of preparation and measurement. We have prepared thin slices of brain tissue suited for indentation measurements on the micrometer scale in a near-native state. Using a scanning force microscope with a spherical indenter of radius similar to 20 mu m we have mapped the effective elastic modulus of rat cerebellum with a spatial resolution of 100 mu m. We found significant differences between white and gray matter, having effective elastic moduli of K=294 +/- 74 and 454 +/- 53 Pa, respectively, at 3 mu m indentation depth (n(g) = 245, n(w)=150 in four animals, p < 0.05; errors are SD). In contrast to many other measurements on larger length scales, our results were constant for indentation depths of 2-4 mu m indicating a regime of linear effective elastic modulus. These data, assessed with a direct mechanical measurement, provide reliable high-resolution information and serve as a quantitative basis for further neuromechanical investigations on the mechanical properties of developing, adult and damaged CNS tissue. (C) 2010 Elsevier Ltd. All rights reserved.