In vivo model systems

Core Facility

Medically relevant laboratory animal science

Various methods are used to investigate specific medical questions from a physical perspective. These methods include in vivo analyses of living tissue and investigations of processes in intact organisms. For this purpose, various model organisms – specifically zebrafish (Danio rerio), clawed frogs (Xenopus laevis) and mice (Mus musculus f. domestica) – are kept in the Core In Vivo Model Systems Facility. In addition to legal requirements, the MPZPM complies with the Max Planck Society’s Declaration on Animal Research, which commits individual scientists to a special responsibility for laboratory animals and the ethical issues associated with research involving living beings.

The Core Facility is the interface between animals and science

Zebrafish, clawed frogs, and mice are kept for research in our Core Facility, whose staff is responsible for the welfare of the laboratory animals in accordance with animal ethics. The main tasks of animal facility management are the humane care and targeted, planned breeding of laboratory animals. A specialist veterinarian works with a team of animal caretakers, technical assistants, and veterinary assistants to ensure the well-being of the animals in accordance with their needs. Monitoring is carried out in cooperation with animal welfare officers. Keeping animals in a manner appropriate to their species and in accordance with animal welfare is not only legally and ethically essential, but also necessary in order to obtain meaningful and reproducible research results later on.

The animal facility is an important point of contact for scientists planning and conducting in vivo experiments and is involved in the planning process from the outset. It provides expert advice on animal experimentation issues and organizes the provision and prophylactic health maintenance of the animals, as well as their care before and during the research work.

The general and specific animal care services go hand in hand with the requirements for effective hygiene management. Veterinary care for the animals, including monitoring their welfare, also includes diagnostics for possible animal diseases. In addition, experimental animal research projects are supported through consultation and supervision. Continuing education and training in laboratory animal science for employees and researchers ensures ongoing compliance with the legally prescribed 3R rules (replace, reduce, refine). The Max Planck Society has introduced the so-called 4R principle for all its institutes in order to enshrine a special focus on human responsibility towards animals.

Micropipette manufacturing

Micropipettes and microinjection needles are required for a wide range of applications in biological and biophysical research. This includes the targeted microinjection of nucleic acids and proteins into cells. Using a micropipette puller, the Core Facility can precisely manufacture its own capillaries for scientists, according to their requirements. This involves heating glass capillaries evenly to a desired temperature using a heating wire and pulling the glass apart under constant tension. By adjusting the temperature and drawing parameters, application-specific pipettes can be produced reproducibly for research from a wide range of glass compositions and sizes.

Contact

Core Facility In vivo Model Systems
Johannes Bachmann

Max-Planck-Zentrum für Physik und Medizin
Kussmaulallee 2
91054 Erlangen, Germany

johannes.bachmann@mpzpm.mpg.de

Core facility leader Johannes Bachmann

Cooperation partners

Graduate Program